Akta pure user manual

Sample loops can be filled manually, via a syringe, or with a sample pump. Akta pure 25 purification system is intended for microgramscale protein purifications in tricorn columns up to milligramscale using hitrap and hiscreen columns. Aktaexplorer am ers ha m bio scie nce am er sham bio ci n a m e r s h a m b io s c ie n c a m e r s h a m b io s c ie box n c e monitor conductivity ph uv detector 3 wavelengths pumps column valves injector sample valve buffers phcell electrode sample holder pump a buffer switching valve outlet valve. Air ventilation uv monitor when using 23 wavelengths pump movement mixer changing valve position fraction collector movements for long term storage. Also i purge d the pump with 100% methanol but everytime. The small diameter, low flow kit manages flows of up to 175 lh using columns typically less than 20 cm. Check that injection valve is set to load column position. Akta pure cytiva, formerly ge healthcare life sciences. Aktaprime plus user manual 11002644 edition aa 9 1introduction this section is an introduction to aktaprime plus, the documentation included and the content in this manual. I tried purging the pump multiple times but no success. Always keep the he inlet above the solution or close the valve. Akta ready is a chromatography system using a disposable flow path. This video show how to set up a two step method in the unicorn software for the akta pure protein.

Apr 01, 2015 issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. Two examples of system configurations for akta pure 25 showing positions of modules on the front panel and flow paths for each. Introduction to this handbook and unicorn aktadesign purification user manual 8 2423 edition ad about this handbook 5. Gloves and protective eyewear should be worn when handling samples and reagents buffers, however it is preferable that the user remove gloves prior to entering commands via the computer keyboard or mouse. Gloves and protective eyewear should be worn when handling samples and reagents buffers, however it is preferable that the user remove gloves prior to entering commands via. Database contains 4 ge akta start manuals available for free online viewing or downloading in pdf. It also includes information on optional equipment. Fraction collector frac950 boyce thompson institute. It also contains an overview of the safety instructions. Easily share your publications and get them in front of issuus. Operate in accordance with local safety instructions. Manual load position of the injection valve that allows the user to load the sample loop with sample by depressing the plunger of inserted syringe full.

Important user information all users must read this entire manual to fully understand the safe use of aktacrossflow. Akta pure 25 new owners intro the exercise below will give a quick demonstration of how easy and intuitive the akta pure 25 will be for you in demonstrating downstream processing to your students. It allows users to speed up process scaleup and cleaning between product. The product must also be used in the same state as it was delivered from ge healthcare. Fraction collector frac950 user manual 181956 edition ae 7 1 introduction 1. It will help if they get w10 with some good dev support when it comes out. This high flow rate is necessary when working with. Jan 09, 2018 akta pure, protein purification your way. Introduction to the akta pure systems hardware and software 3. The akta purifier 100 is perfect for application spanning a wide range of wavelengths the akta purifier 100 allows for rate flows of up to 100 ml per minute fast and easy start to purification work preprogrammed application protocols and method templates speed and reliability for every run fast optimisation and peak identificationaccurate result comparisons and peakfractionation column. Fraction collector frac950 user manual 181956 edition ae 9 1.

Fplc standard operating procedure akta purifier system page page 2 of 9 check there is sufficient volumes of buffers, mq and etoh 20% for the coming runs. The system consists of the akta pcc instrument and unicorn system control software. Scope of this manual the operating instructions cover akta pure 25 and akta pure 150 instruments, using previously created methods in unicorn software. This automatic configuration protects the system from damaging events including over pressure. Fully scalable, the unicorn platform is suitable for use within smallscale research all the way to fullscale manufacturing. Troubleshooting check that the conductivity signal is stable before sample injection check the gradient performance possible to identify when the buffer has been completely exchangedwashed out. In the unicorn software, prompt the system control window. Check valve is clogged or damaged, causing leakage. Twostep purification with akta pure, or akta avant, using loop. Technical details about the instrument and the individual modules are found in akta pure user manual. It is intended to be operated as an integrated part of an aktadesign chromatography system running unicorn software. Unicorn system control software provides builtin knowledge for planning and controlling runs, as well as analyzing results.

Akta fplc system operators manual marshall scientific by. The instrument offers easy access to working areas using a swivel foot, and has a modular design, with all valves, monitors, and columns mounted on the wet side of. Data file 28915986 ad readytoprocess akta ready system fig 1. Exterior design akta pure has a modular design, with all liquid handling modules placed on the exterior of the instrument. Akta pure chromatography system is a highly versatile, modular system with a number of design features to facilitate reliable purification.

The system is designed for process development, process scaleup and scale. Length mounted mm label description tubing b1andb2 mounted b1b7 inletstoinletvalveb fep, o. Ensure your akta system is operating at peakperformance by. Falls unicorn keine verbindung mit dem ausgewahlten gerat herstellen kann, siehe fehlersuche in akta pure user manual. Function the fraction collector collects fractions from akta pure purification runs. The product fulfills valid directives and standards when used within the conditions specified in the user manual. Ge akta explorer certified fplc system gmi trusted lab.

Unicorn 7 features an intuitive interface for creating methods by dragging and dropping predefined phases for a wide range of techniques. Full programming control is available for almost any imaginable purification. Twostep purification with akta pure, or akta avant, using loop collection cue card contents principles 2 setup for multistep purification 2 unicorn methods 3 how to add user defined phases to a method 3 description of the user defined phase in method one 4 edit sample application phase in method two 5 recommendations 6 introduction this cue card describes how to configure akta pure. Akta explorer systems include an extensive column library with details of run parameters for each prepacked column. Opening the unicorn software windows for manual control and programmable features purgingpriming pumps and tubing. Ge akta pure operating instructions manual pdf download.

I recently ran many samples on our akta pure without any intermittent cleaning. The software can control chromatography, bioreactor, and filtration systems fig 1. Systems provide more control than manual purification because of the ability to automatically control the flow rate and monitor the progress of the purification as well as to make controlled gradients and automatically collect fractions. Unicorn 7 cytiva, formerly ge healthcare life sciences. Akta fplc system operators manual marshall scientific. The aim of this tutorial is to demonstrate the use of the ubiquitous akta systems and prepacked hitrap chromatography media for rapid, mediumscale protein purification. Unicorn efficient method creation is often about finding useful tips on how to develop methods easy to maintain, reuse, and share. A full description of how to operate unicorn is found in the unicorn user manual. For fast protein liquid chromatography of tens of grams of protein, akta pure 150 can be used with flow rates up to 150 mlmin. Cytiva formerly ge healthcare life sciences 16,785 views. Aug 24, 2018 if you are tied to your akta protein purification system during manual runs, learn how to get started with quick method creation using unicorn control software.

Using the akta prime plus october 22, 2012 3 j e n n i f e r a r r u d a attaching the column and washing the ethanol from the column. Once the lines have been washed with water, you will need to start a. Once the lines have been washed with water, you will need to start a manual run before attaching your column to the system. Akta ready system 1 inlet manifold, 2 pump tubing, and 3 main flow path. Priming system and sample pumps for aktapure and aktaavant. When the user selects a column, the akta explorer instinctively sets the column volume, flow rate and pressure limit parameters into the method template. Akta pure user manual ge healthcare life sciences manualzz. For further details see akta avant user manual and akta pure user manual. Technical details are found in the akta pure user manual and akta avant user manual.

Ge pharmacia akta purifier 100 gmi trusted laboratory. How can i get rid of air in an akta purifier inlet tube. A a basic system configuration for convenient protein purification. Get familiarized with its use and have its instruction manual handy. All users must read this entire manual to fully understand the safe use of akta. Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. User manuals, guides and specifications for your ge akta start laboratory equipment. Do not use in a dusty atmosphere or close to spraying water. File containing module user manuals and component instructions for aktafplc equipment pump p920 user manual monitor upc900 user manual.

A simple, yet flexible, control software for akta protein purification systems. Aktaexplorer am ers ha m bio scie nce am er sham bio ci n a m e r s h a m b io s c i en c a m e r s h a m b io s c i e n c e akta system training guide explorer 100 10. The system consists of the akta pure instrument and. If the leakage persists, replace the assembly according to instructions in the pump p960 user manual. Aktacrossflow is intended for laboratory use only, not for clinical or in vitro use, or for diagnostic purposes. Part function 1 multimodulepanel 2 inletvalve 3 pumprinsingliquidtube 4 systempumpb 5 pressuremonitor 6 systempumpa 7 mixer 8 outletvalve 9 injectionvalve. Tubing kit changes aktapurifier 100 high flow at delivery 0. For further details see akta avant user manual and akta pure user manual references on page 14. Akta pure is a flexible and intuitive purification system for proteins, peptides, and nucleic acids from microgram levels to tens of grams of target product in research applications. Akta pure, protein purification your way april scharfstein. Akta pure is a flexible chromatography system for the reliable purification of proteins, peptides, and nucleic acids at laboratory scale. Important user information this document is a handbook with complementing information to the operating instructions. Introduction to column resins and chromatography separation methods 4.

Weitere details finden sie in akta pure user manual. All users must read the safety instructions in the aktapilot operating instructions to fully understand the safe use of the aktapilot system, before installing, using, or maintaining the system. Akta pure 25, designed for a broad range of applications and purification tasks in a multiuser environment, and. Protein purification laboratory of molecular biology.